Technical Details
Human IL-6, accession # P05231, Pro29-Met212
Lyophilized from a 0.2 µm-filtered solution containing 1.5 mM potassium phosphate, 8.1 mM sodium phosphate, 2.7 mM potassium chloride and 137 mM sodium chloride, pH 7.5
Both product grades are produced under the same conditions in a GMP facility, ensuring an equal product quality and performance. We offer a more comprehensive QC testing including tighter specifications and documentation for our GMP products: Preclinical vs GMP.
| Preclinical | GMP | |
| Molecular weight | 21.9 kDa | 21.9 kDa |
| Purity | ≥ 95% as determined by SDS-PAGE | ≥ 97% as determined by SDS-PAGE |
| Activity | ≥ 80 x 106 IU/mg, calibrated against NIBSC #89/548 Measured in a cell proliferation assay using an IL-6-dependent cell line, B9 | 80 – 250 x 106 IU/mg, calibrated against NIBSC #89/548 Measured in a cell proliferation assay using an IL-6-dependent cell line, B9 Batch specific activity on CoA |
| Endotoxin level | < 1000 EU/mg | ≤ 50 EU/mg |
| Intended use | Intended for preclinical ex vivo use. Not intended for therapeutic use. | Intended for clinical ex vivo use. Not intended for human in vivo application. |
Handling Instructions
Recommended in sterile water to a final concentration of 250 µg/ml for 50 µg vials and for 1 mg vials. We recommend to not vortex the solution. The solution can be turbid, this however does not influence the activity.
Ambient temperature. Please refer to Technote to learn more about our shipment validation procedure.
≥ 6 months from date of shipping. Please refer to Certificate of Analysis for the exact expiry date.
Store lyophilized cytokine at -20°C to -80°C.
Store a 100 µg/ml reconstituted cytokine solution:
• 4 weeks at 2°C to 8°C under sterile conditions after reconstitution. Store in the original container.
• 4 months at -20°C to -80°C under sterile conditions after reconstitution. Store in 60 µl aliquots in polypropylene cryogenic vials.
Avoid repeated freeze/thaw cycles.
